Large-volume en-bloc staining for electron microscopy-based connectomics

Yunfeng Hua (), Philip Laserstein and Moritz Helmstaedter ()
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Yunfeng Hua: Max Planck Institute of Brain Research
Philip Laserstein: Max Planck Institute of Brain Research
Moritz Helmstaedter: Max Planck Institute of Brain Research

Nature Communications, 2015, vol. 6, issue 1, 1-7

Abstract: Abstract Large-scale connectomics requires dense staining of neuronal tissue blocks for electron microscopy (EM). Here we report a large-volume dense en-bloc EM staining protocol that overcomes the staining gradients, which so far substantially limited the reconstructable volumes in three-dimensional (3D) EM. Our protocol provides densely reconstructable tissue blocks from mouse neocortex sized at least 1 mm in diameter. By relaxing the constraints on precise topographic sample targeting, it makes the correlated functional and structural analysis of neuronal circuits realistic.

Date: 2015
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DOI: 10.1038/ncomms8923

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