Mapping molecules in scanning far-field fluorescence nanoscopy

Haisen Ta, Jan Keller, Markus Haltmeier, Sinem K. Saka, Jürgen Schmied, Felipe Opazo, Philip Tinnefeld, Axel Munk and Stefan W. Hell ()
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Haisen Ta: Max Planck Institute for Biophysical Chemistry
Jan Keller: Max Planck Institute for Biophysical Chemistry
Markus Haltmeier: Statistical Inverse Problems in Biophysics Group, Max Planck Institute for Biophysical Chemistry
Sinem K. Saka: University Medical Center Göttingen
Jürgen Schmied: NanoBioSciences Group, Institute of Physical and Theoretical Chemistry, Braunschweig University of Technology
Felipe Opazo: University Medical Center Göttingen
Philip Tinnefeld: NanoBioSciences Group, Institute of Physical and Theoretical Chemistry, Braunschweig University of Technology
Axel Munk: Statistical Inverse Problems in Biophysics Group, Max Planck Institute for Biophysical Chemistry
Stefan W. Hell: Max Planck Institute for Biophysical Chemistry

Nature Communications, 2015, vol. 6, issue 1, 1-7

Abstract: Abstract In fluorescence microscopy, the distribution of the emitting molecule number in space is usually obtained by dividing the measured fluorescence by that of a single emitter. However, the brightness of individual emitters may vary strongly in the sample or be inaccessible. Moreover, with increasing (super-) resolution, fewer molecules are found per pixel, making this approach unreliable. Here we map the distribution of molecules by exploiting the fact that a single molecule emits only a single photon at a time. Thus, by analysing the simultaneous arrival of multiple photons during confocal imaging, we can establish the number and local brightness of typically up to 20 molecules per confocal (diffraction sized) recording volume. Subsequent recording by stimulated emission depletion microscopy provides the distribution of the number of molecules with subdiffraction resolution. The method is applied to mapping the three-dimensional nanoscale organization of internalized transferrin receptors on human HEK293 cells.

Date: 2015
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DOI: 10.1038/ncomms8977

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