A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis

Joon-Yung Cha, Woe-Yeon Kim, Sun Bin Kang, Jeong Im Kim, Dongwon Baek, In Jung Jung, Mi Ri Kim, Ning Li, Hyun-Jin Kim, Masatoshi Nakajima, Tadao Asami, Jamal S. M. Sabir, Hyeong Cheol Park, Sang Yeol Lee, Hans J. Bohnert, Ray A. Bressan, Jose M. Pardo and Dae-Jin Yun ()
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Joon-Yung Cha: PMBBRC & IALS, Gyeongsang National University
Woe-Yeon Kim: PMBBRC & IALS, Gyeongsang National University
Sun Bin Kang: PMBBRC & IALS, Gyeongsang National University
Jeong Im Kim: Purdue University
Dongwon Baek: PMBBRC & IALS, Gyeongsang National University
In Jung Jung: PMBBRC & IALS, Gyeongsang National University
Mi Ri Kim: PMBBRC & IALS, Gyeongsang National University
Ning Li: PMBBRC & IALS, Gyeongsang National University
Hyun-Jin Kim: PMBBRC & IALS, Gyeongsang National University
Masatoshi Nakajima: The University of Tokyo
Tadao Asami: The University of Tokyo
Jamal S. M. Sabir: Biotechnology Research Group, Faculty of Science, King Abdulaziz University
Hyeong Cheol Park: National Institute of Ecology
Sang Yeol Lee: PMBBRC & IALS, Gyeongsang National University
Hans J. Bohnert: Biotechnology Research Group, Faculty of Science, King Abdulaziz University
Ray A. Bressan: Biotechnology Research Group, Faculty of Science, King Abdulaziz University
Jose M. Pardo: Instituto de Recursos Naturales y Agrobiologia, Consejo Superior de Investigaciones Cientificas
Dae-Jin Yun: PMBBRC & IALS, Gyeongsang National University

Nature Communications, 2015, vol. 6, issue 1, 1-13

Abstract: Abstract YUCCA (YUC) proteins constitute a family of flavin monooxygenases (FMOs), with an important role in auxin (IAA) biosynthesis. Here we report that Arabidopsis plants overexpressing YUC6 display enhanced IAA-related phenotypes and exhibit improved drought stress tolerance, low rate of water loss and controlled ROS accumulation under drought and oxidative stresses. Co-overexpression of an IAA-conjugating enzyme reduces IAA levels but drought stress tolerance is unaffected, indicating that the stress-related phenotype is not based on IAA overproduction. YUC6 contains a previously unrecognized FAD- and NADPH-dependent thiol-reductase activity (TR) that overlaps with the FMO domain involved in IAA biosynthesis. Mutation of a conserved cysteine residue (Cys-85) preserves FMO but suppresses TR activity and stress tolerance, whereas mutating the FAD- and NADPH-binding sites, that are common to TR and FMO domains, abolishes all outputs. We provide a paradigm for a single protein playing a dual role, regulating plant development and conveying stress defence responses.

Date: 2015
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DOI: 10.1038/ncomms9041

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