High-resolution analysis of the human T-cell receptor repertoire

Eliana Ruggiero, Jan P. Nicolay, Raffaele Fronza, Anne Arens, Anna Paruzynski, Ali Nowrouzi, Gökçe Ürenden, Christina Lulay, Sven Schneider, Sergij Goerdt, Hanno Glimm, Peter H. Krammer, Manfred Schmidt and Christof von Kalle ()
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Eliana Ruggiero: National Center for Tumor Diseases and German Cancer Research Center
Jan P. Nicolay: German Cancer Research Center
Raffaele Fronza: National Center for Tumor Diseases and German Cancer Research Center
Anne Arens: National Center for Tumor Diseases and German Cancer Research Center
Anna Paruzynski: National Center for Tumor Diseases and German Cancer Research Center
Ali Nowrouzi: National Center for Tumor Diseases and German Cancer Research Center
Gökçe Ürenden: National Center for Tumor Diseases and German Cancer Research Center
Christina Lulay: National Center for Tumor Diseases and German Cancer Research Center
Sven Schneider: Institute for Clinical Chemistry, University Medical Center, Ruprecht-Karls-University of Heidelberg
Sergij Goerdt: Venereology and Allergology, University Medical Center
Hanno Glimm: National Center for Tumor Diseases and German Cancer Research Center
Peter H. Krammer: German Cancer Research Center
Manfred Schmidt: National Center for Tumor Diseases and German Cancer Research Center
Christof von Kalle: National Center for Tumor Diseases and German Cancer Research Center

Nature Communications, 2015, vol. 6, issue 1, 1-7

Abstract: Abstract Unbiased dissection of T-cell receptor (TCR) repertoire diversity at the nucleotide level could provide important insights into human immunity. Here we show that TCR ligation-anchored-magnetically captured PCR (TCR-LA-MC PCR) identifies TCR α- and β-chain diversity without sequence-associated or quantitative restrictions in healthy and diseased conditions. TCR-LA-MC PCR identifies convergent recombination events, classifies different stages of cutaneous T-cell lymphoma in vivo and demonstrates TCR reactivation after in vitro cytomegalovirus stimulation. TCR-LA-MC PCR allows ultra-deep data access to both physiological TCR diversity and mechanisms influencing clonality in all clinical settings with restricted or distorted TCR repertoires.

Date: 2015
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DOI: 10.1038/ncomms9081

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