An Arabidopsis PWI and RRM motif-containing protein is critical for pre-mRNA splicing and ABA responses

Xiangqiang Zhan (), Bilian Qian, Fengqiu Cao, Wenwu Wu, Lan Yang, Qingmei Guan, Xianbin Gu, Pengcheng Wang, Temiloluwa A. Okusolubo, Stephanie L. Dunn, Jian-Kang Zhu and Jianhua Zhu ()
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Xiangqiang Zhan: Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences
Bilian Qian: University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA
Fengqiu Cao: Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences
Wenwu Wu: Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences
Lan Yang: Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences
Qingmei Guan: University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA
Xianbin Gu: University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA
Pengcheng Wang: Purdue University
Temiloluwa A. Okusolubo: University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA
Stephanie L. Dunn: University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA
Jian-Kang Zhu: Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences
Jianhua Zhu: University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA

Nature Communications, 2015, vol. 6, issue 1, 1-12

Abstract: Abstract The phytohormone abscisic acid (ABA) is important for growth, development and stress responses in plants. Recent research has identified ABA receptors and signalling components that regulate seed germination and stomatal closure. However, proteins that regulate ABA signalling remain poorly understood. Here we use a forward-genetic screen to identify rbm25-1 and rbm25-2, two Arabidopsis mutants with increased sensitivity to growth inhibition by ABA. Using RNA-seq, we found that RBM25 controls the splicing of many pre-mRNAs. The protein phosphatase 2C HAB1, a critical component in ABA signalling, shows a dramatic defect in pre-mRNA splicing in rbm25 mutants. Ectopic expression of a HAB1 complementary DNA derived from wild-type mRNAs partially suppresses the rbm25-2 mutant phenotype. We suggest that RNA splicing is of particular importance for plant response to ABA and that the splicing factor RBM25 has a critical role in this response.

Date: 2015
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DOI: 10.1038/ncomms9139

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