Chamber identity programs drive early functional partitioning of the heart

Christian Mosimann, Daniela Panáková, Andreas A. Werdich, Gabriel Musso, Alexa Burger, Katy L. Lawson, Logan A. Carr, Kathleen R. Nevis, M. Khaled Sabeh, Yi Zhou, Alan J. Davidson, Anthony DiBiase, Caroline E. Burns, C. Geoffrey Burns, Calum A. MacRae () and Leonard I. Zon ()
Additional contact information
Christian Mosimann: Howard Hughes Medical Institute
Daniela Panáková: Brigham and Women's Hospital, Harvard Medical School
Andreas A. Werdich: Brigham and Women's Hospital, Harvard Medical School
Gabriel Musso: Brigham and Women's Hospital, Harvard Medical School
Alexa Burger: Institute of Molecular Life Sciences (IMLS), University of Zürich
Katy L. Lawson: Howard Hughes Medical Institute
Logan A. Carr: Howard Hughes Medical Institute
Kathleen R. Nevis: Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School
M. Khaled Sabeh: Brigham and Women's Hospital, Harvard Medical School
Yi Zhou: Howard Hughes Medical Institute
Alan J. Davidson: Howard Hughes Medical Institute
Anthony DiBiase: Howard Hughes Medical Institute
Caroline E. Burns: Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School
C. Geoffrey Burns: Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School
Calum A. MacRae: Brigham and Women's Hospital, Harvard Medical School
Leonard I. Zon: Howard Hughes Medical Institute

Nature Communications, 2015, vol. 6, issue 1, 1-10

Abstract: Abstract The vertebrate heart muscle (myocardium) develops from the first heart field (FHF) and expands by adding second heart field (SHF) cells. While both lineages exist already in teleosts, the primordial contributions of FHF and SHF to heart structure and function remain incompletely understood. Here we delineate the functional contribution of the FHF and SHF to the zebrafish heart using the cis-regulatory elements of the draculin (drl) gene. The drl reporters initially delineate the lateral plate mesoderm, including heart progenitors. Subsequent myocardial drl reporter expression restricts to FHF descendants. We harnessed this unique feature to uncover that loss of tbx5a and pitx2 affect relative FHF versus SHF contributions to the heart. High-resolution physiology reveals distinctive electrical properties of each heart field territory that define a functional boundary within the single zebrafish ventricle. Our data establish that the transcriptional program driving cardiac septation regulates physiologic ventricle partitioning, which successively provides mechanical advantages of sequential contraction.

Date: 2015
References: Add references at CitEc
Citations: View citations in EconPapers (3)

Downloads: (external link)
https://www.nature.com/articles/ncomms9146 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9146

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms9146

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().