Development of high-yield influenza A virus vaccine viruses

Jihui Ping, Tiago J.S. Lopes, Chairul A. Nidom, Elodie Ghedin, Catherine A. Macken, Adam Fitch, Masaki Imai, Eileen A. Maher, Gabriele Neumann and Yoshihiro Kawaoka ()
Additional contact information
Jihui Ping: School of Veterinary Medicine, Influenza Research Institute, University of Wisconsin-Madison
Tiago J.S. Lopes: School of Veterinary Medicine, Influenza Research Institute, University of Wisconsin-Madison
Chairul A. Nidom: Avian Influenza-Zoonosis Research Center, Airlangga University
Elodie Ghedin: New York University
Catherine A. Macken: Bioinformatics Institute, University of Auckland
Adam Fitch: University of Pittsburgh School of Medicine
Masaki Imai: School of Veterinary Medicine, Influenza Research Institute, University of Wisconsin-Madison
Eileen A. Maher: School of Veterinary Medicine, Influenza Research Institute, University of Wisconsin-Madison
Gabriele Neumann: School of Veterinary Medicine, Influenza Research Institute, University of Wisconsin-Madison
Yoshihiro Kawaoka: School of Veterinary Medicine, Influenza Research Institute, University of Wisconsin-Madison

Nature Communications, 2015, vol. 6, issue 1, 1-15

Abstract: Abstract Vaccination is one of the most cost-effective ways to prevent infection. Influenza vaccines propagated in cultured cells are approved for use in humans, but their yields are often suboptimal. Here, we screened A/Puerto Rico/8/34 (PR8) virus mutant libraries to develop vaccine backbones (defined here as the six viral RNA segments not encoding haemagglutinin and neuraminidase) that support high yield in cell culture. We also tested mutations in the coding and regulatory regions of the virus, and chimeric haemagglutinin and neuraminidase genes. A combination of high-yield mutations from these screens led to a PR8 backbone that improved the titres of H1N1, H3N2, H5N1 and H7N9 vaccine viruses in African green monkey kidney and Madin–Darby canine kidney cells. This PR8 backbone also improves titres in embryonated chicken eggs, a common propagation system for influenza viruses. This PR8 vaccine backbone thus represents an advance in seasonal and pandemic influenza vaccine development.

Date: 2015
References: Add references at CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.nature.com/articles/ncomms9148 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9148

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms9148

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().