mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3

Anna Gebhardt, Matthias Habjan, Christian Benda, Arno Meiler, Darya A. Haas, Marco Y. Hein, Angelika Mann, Matthias Mann, Bianca Habermann and Andreas Pichlmair ()
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Anna Gebhardt: Innate Immunity Laboratory, Max-Planck Institute of Biochemistry
Matthias Habjan: Innate Immunity Laboratory, Max-Planck Institute of Biochemistry
Christian Benda: Max-Planck Institute of Biochemistry
Arno Meiler: Innate Immunity Laboratory, Max-Planck Institute of Biochemistry
Darya A. Haas: Innate Immunity Laboratory, Max-Planck Institute of Biochemistry
Marco Y. Hein: Max-Planck Institute of Biochemistry
Angelika Mann: Innate Immunity Laboratory, Max-Planck Institute of Biochemistry
Matthias Mann: Max-Planck Institute of Biochemistry
Bianca Habermann: Bioinformatics Core Facility, Max-Planck Institute of Biochemistry
Andreas Pichlmair: Innate Immunity Laboratory, Max-Planck Institute of Biochemistry

Nature Communications, 2015, vol. 6, issue 1, 1-13

Abstract: Abstract The flow of genetic information from DNA to protein requires polymerase-II-transcribed RNA characterized by the presence of a 5′-cap. The cap-binding complex (CBC), consisting of the nuclear cap-binding protein (NCBP) 2 and its adaptor NCBP1, is believed to bind all capped RNA and to be necessary for its processing and intracellular localization. Here we show that NCBP1, but not NCBP2, is required for cell viability and poly(A) RNA export. We identify C17orf85 (here named NCBP3) as a cap-binding protein that together with NCBP1 forms an alternative CBC in higher eukaryotes. NCBP3 binds mRNA, associates with components of the mRNA processing machinery and contributes to poly(A) RNA export. Loss of NCBP3 can be compensated by NCBP2 under steady-state conditions. However, NCBP3 becomes pivotal under stress conditions, such as virus infection. We propose the existence of an alternative CBC involving NCBP1 and NCBP3 that plays a key role in mRNA biogenesis.

Date: 2015
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DOI: 10.1038/ncomms9192

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