TORC1 controls G1–S cell cycle transition in yeast via Mpk1 and the greatwall kinase pathway

Marta Moreno-Torres, Malika Jaquenoud and Claudio De Virgilio ()
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Marta Moreno-Torres: University of Fribourg
Malika Jaquenoud: University of Fribourg
Claudio De Virgilio: University of Fribourg

Nature Communications, 2015, vol. 6, issue 1, 1-10

Abstract: Abstract The target of rapamycin complex 1 (TORC1) pathway couples nutrient, energy and hormonal signals with eukaryotic cell growth and division. In yeast, TORC1 coordinates growth with G1–S cell cycle progression, also coined as START, by favouring the expression of G1 cyclins that activate cyclin-dependent protein kinases (CDKs) and by destabilizing the CDK inhibitor Sic1. Following TORC1 downregulation by rapamycin treatment or nutrient limitation, clearance of G1 cyclins and C-terminal phosphorylation of Sic1 by unknown protein kinases are both required for Sic1 to escape ubiquitin-dependent proteolysis prompted by its flagging via the SCFCdc4 (Skp1/Cul1/F-box protein) ubiquitin ligase complex. Here we show that the stabilizing phosphorylation event within the C-terminus of Sic1 requires stimulation of the mitogen-activated protein kinase, Mpk1, and inhibition of the Cdc55 protein phosphatase 2A (PP2ACdc55) by greatwall kinase-activated endosulfines. Thus, Mpk1 and the greatwall kinase pathway serve TORC1 to coordinate the phosphorylation status of Sic1 and consequently START with nutrient availability.

Date: 2015
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DOI: 10.1038/ncomms9256

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