Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1

Lorenza Ciani, Aude Marzo, Kieran Boyle, Eleanna Stamatakou, Douglas M. Lopes, Derek Anane, Faye McLeod, Silvana B. Rosso, Alasdair Gibb () and Patricia C. Salinas ()
Additional contact information
Lorenza Ciani: University College London
Aude Marzo: University College London
Kieran Boyle: University College London
Eleanna Stamatakou: University College London
Douglas M. Lopes: University College London
Derek Anane: University College London
Faye McLeod: University College London
Silvana B. Rosso: University College London
Alasdair Gibb: Physiology and Pharmacology, University College London
Patricia C. Salinas: University College London

Nature Communications, 2015, vol. 6, issue 1, 1-13

Abstract: Abstract The functional assembly of the synaptic release machinery is well understood; however, how signalling factors modulate this process remains unknown. Recent studies suggest that Wnts play a role in presynaptic function. To examine the mechanisms involved, we investigated the interaction of release machinery proteins with Dishevelled-1 (Dvl1), a scaffold protein that determines the cellular locale of Wnt action. Here we show that Dvl1 directly interacts with Synaptotagmin-1 (Syt-1) and indirectly with the SNARE proteins SNAP25 and Syntaxin (Stx-1). Importantly, the interaction of Dvl1 with Syt-1, which is regulated by Wnts, modulates neurotransmitter release. Moreover, presynaptic terminals from Wnt signalling-deficient mice exhibit reduced release probability and are unable to sustain high-frequency release. Consistently, the readily releasable pool size and formation of SNARE complexes are reduced. Our studies demonstrate that Wnt signalling tunes neurotransmitter release and identify Syt-1 as a target for modulation by secreted signalling proteins.

Date: 2015
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms9302 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9302

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms9302

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().