Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation

Adeel Asghar, Audrey Lajeunesse, Kalyan Dulla, Guillaume Combes, Philippe Thebault, Erich A. Nigg and Sabine Elowe ()
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Adeel Asghar: Faculty of Medicine, Université Laval
Audrey Lajeunesse: Faculty of Medicine, Université Laval
Kalyan Dulla: ProQR Therapeutics N.V.
Guillaume Combes: Faculty of Medicine, Université Laval
Philippe Thebault: Mother and Youth Health, Centre de recherche du Centre Hospitalier Universitaire de Québec
Erich A. Nigg: Biozentrum, University of Basel, Klingelbergstrasse 50/70, Basel CH-4056, Switzerland
Sabine Elowe: Faculty of Medicine, Université Laval

Nature Communications, 2015, vol. 6, issue 1, 1-14

Abstract: Abstract During mitosis, Bub1 kinase phosphorylates histone H2A-T120 to promote centromere sister chromatid cohesion through recruitment of shugoshin (Sgo) proteins. The regulation and dynamics of H2A-T120 phosphorylation are poorly understood. Using quantitative phosphoproteomics we show that Bub1 is autophosphorylated at numerous sites. We confirm mitosis-specific autophosphorylation of a several residues and show that Bub1 activation is primed in interphase but fully achieved only in mitosis. Mutation of a single autophosphorylation site T589 alters kinetochore turnover of Bub1 and results in uniform H2A-T120 phosphorylation and Sgo recruitment along chromosome arms. Consequently, improper sister chromatid resolution and chromosome segregation errors are observed. Kinetochore tethering of Bub1-T589A refocuses H2A-T120 phosphorylation and Sgo1 to centromeres. Recruitment of the Bub1-Bub3-BubR1 axis to kinetochores has recently been extensively studied. Our data provide novel insight into the regulation and kinetochore residency of Bub1 and indicate that its localization is dynamic and tightly controlled through feedback autophosphorylation.

Date: 2015
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DOI: 10.1038/ncomms9364

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