Wall mechanics and exocytosis define the shape of growth domains in fission yeast

Juan F. Abenza (), Etienne Couturier, James Dodgson, Johanna Dickmann, Anatole Chessel, Jacques Dumais () and Rafael E. Carazo Salas ()
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Juan F. Abenza: University of Cambridge
Etienne Couturier: Universidad de Santiago de Chile
James Dodgson: University of Cambridge
Johanna Dickmann: University of Cambridge
Anatole Chessel: University of Cambridge
Jacques Dumais: Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez
Rafael E. Carazo Salas: University of Cambridge

Nature Communications, 2015, vol. 6, issue 1, 1-13

Abstract: Abstract The amazing structural variety of cells is matched only by their functional diversity, and reflects the complex interplay between biochemical and mechanical regulation. How both regulatory layers generate specifically shaped cellular domains is not fully understood. Here, we report how cell growth domains are shaped in fission yeast. Based on quantitative analysis of cell wall expansion and elasticity, we develop a model for how mechanics and cell wall assembly interact and use it to look for factors underpinning growth domain morphogenesis. Surprisingly, we find that neither the global cell shape regulators Cdc42-Scd1-Scd2 nor the major cell wall synthesis regulators Bgs1-Bgs4-Rgf1 are reliable predictors of growth domain geometry. Instead, their geometry can be defined by cell wall mechanics and the cortical localization pattern of the exocytic factors Sec6-Syb1-Exo70. Forceful re-directioning of exocytic vesicle fusion to broader cortical areas induces proportional shape changes to growth domains, demonstrating that both features are causally linked.

Date: 2015
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DOI: 10.1038/ncomms9400

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