A RIAM/lamellipodin–talin–integrin complex forms the tip of sticky fingers that guide cell migration

Frederic Lagarrigue, Praju Vikas Anekal, Ho-Sup Lee, Alexia I. Bachir, Jailal N. Ablack, Alan F. Horwitz and Mark H. Ginsberg ()
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Frederic Lagarrigue: University of California San Diego
Praju Vikas Anekal: University of California San Diego
Ho-Sup Lee: University of California San Diego
Alexia I. Bachir: University of Virginia
Jailal N. Ablack: University of California San Diego
Alan F. Horwitz: University of Virginia
Mark H. Ginsberg: University of California San Diego

Nature Communications, 2015, vol. 6, issue 1, 1-13

Abstract: Abstract The leading edge of migrating cells contains rapidly translocating activated integrins associated with growing actin filaments that form ‘sticky fingers’ to sense extracellular matrix and guide cell migration. Here we utilized indirect bimolecular fluorescence complementation to visualize a molecular complex containing a Mig-10/RIAM/lamellipodin (MRL) protein (Rap1-GTP-interacting adaptor molecule (RIAM) or lamellipodin), talin and activated integrins in living cells. This complex localizes at the tips of growing actin filaments in lamellipodial and filopodial protrusions, thus corresponding to the tips of the ‘sticky fingers.’ Formation of the complex requires talin to form a bridge between the MRL protein and the integrins. Moreover, disruption of the MRL protein–integrin–talin (MIT) complex markedly impairs cell protrusion. These data reveal the molecular basis of the formation of ‘sticky fingers’ at the leading edge of migrating cells and show that an MIT complex drives these protrusions.

Date: 2015
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DOI: 10.1038/ncomms9492

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