SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy

Gražvydas Lukinavičius, Claudia Blaukopf, Elias Pershagen, Alberto Schena, Luc Reymond, Emmanuel Derivery, Marcos Gonzalez-Gaitan, Elisa D’Este, Stefan W. Hell, Daniel Wolfram Gerlich () and Kai Johnsson ()
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Gražvydas Lukinavičius: Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology
Claudia Blaukopf: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter Campus (VBC)
Elias Pershagen: Uppsala University
Alberto Schena: Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology
Luc Reymond: Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology
Emmanuel Derivery: NCCR in Chemical Biology, University of Geneva
Marcos Gonzalez-Gaitan: NCCR in Chemical Biology, University of Geneva
Elisa D’Este: Max-Planck-Institute for Biophysical Chemistry
Stefan W. Hell: Max-Planck-Institute for Biophysical Chemistry
Daniel Wolfram Gerlich: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter Campus (VBC)
Kai Johnsson: Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology

Nature Communications, 2015, vol. 6, issue 1, 1-7

Abstract: Abstract Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR–Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.

Date: 2015
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DOI: 10.1038/ncomms9497

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