S-nitrosylation triggers ABI5 degradation to promote seed germination and seedling growth

Pablo Albertos, María C. Romero-Puertas, Kiyoshi Tatematsu, Isabel Mateos, Inmaculada Sánchez-Vicente, Eiji Nambara and Oscar Lorenzo ()
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Pablo Albertos: Dpto. de Microbiología y Genética, Instituto Hispano-Luso de Investigaciones Agrarias (CIALE), Facultad de Biología, Universidad de Salamanca
María C. Romero-Puertas: Dpto. de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas
Kiyoshi Tatematsu: Laboratory of Plant Organ Development, National Institute for Basic Biology
Isabel Mateos: Dpto. de Microbiología y Genética, Instituto Hispano-Luso de Investigaciones Agrarias (CIALE), Facultad de Biología, Universidad de Salamanca
Inmaculada Sánchez-Vicente: Dpto. de Microbiología y Genética, Instituto Hispano-Luso de Investigaciones Agrarias (CIALE), Facultad de Biología, Universidad de Salamanca
Eiji Nambara: University of Toronto
Oscar Lorenzo: Dpto. de Microbiología y Genética, Instituto Hispano-Luso de Investigaciones Agrarias (CIALE), Facultad de Biología, Universidad de Salamanca

Nature Communications, 2015, vol. 6, issue 1, 1-10

Abstract: Abstract Plant survival depends on seed germination and progression through post-germinative developmental checkpoints. These processes are controlled by the stress phytohormone abscisic acid (ABA). ABA regulates the basic leucine zipper transcriptional factor ABI5, a central hub of growth repression, while the reactive nitrogen molecule nitric oxide (NO) counteracts ABA during seed germination. However, the molecular mechanisms by which seeds sense more favourable conditions and start germinating have remained elusive. Here we show that ABI5 promotes growth via NO, and that ABI5 accumulation is altered in genetic backgrounds with impaired NO homeostasis. S-nitrosylation of ABI5 at cysteine-153 facilitates its degradation through CULLIN4-based and KEEP ON GOING E3 ligases, and promotes seed germination. Conversely, mutation of ABI5 at cysteine-153 deregulates protein stability and inhibition of seed germination by NO depletion. These findings suggest an inverse molecular link between NO and ABA hormone signalling through distinct posttranslational modifications of ABI5 during early seedling development.

Date: 2015
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DOI: 10.1038/ncomms9669

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