Formin and capping protein together embrace the actin filament in a ménage à trois

Shashank Shekhar, Mikael Kerleau, Sonja Kühn, Julien Pernier, Guillaume Romet-Lemonne, Antoine Jégou () and Marie-France Carlier ()
Additional contact information
Shashank Shekhar: Cytoskeleton Dynamics and Cell Motility, Biophysics and Structural Biology, I2BC, CNRS
Mikael Kerleau: Cytoskeleton Dynamics and Cell Motility, Biophysics and Structural Biology, I2BC, CNRS
Sonja Kühn: Cytoskeleton Dynamics and Cell Motility, Biophysics and Structural Biology, I2BC, CNRS
Julien Pernier: Cytoskeleton Dynamics and Cell Motility, Biophysics and Structural Biology, I2BC, CNRS
Guillaume Romet-Lemonne: Cytoskeleton Dynamics and Cell Motility, Biophysics and Structural Biology, I2BC, CNRS
Antoine Jégou: Cytoskeleton Dynamics and Cell Motility, Biophysics and Structural Biology, I2BC, CNRS
Marie-France Carlier: Cytoskeleton Dynamics and Cell Motility, Biophysics and Structural Biology, I2BC, CNRS

Nature Communications, 2015, vol. 6, issue 1, 1-12

Abstract: Abstract Proteins targeting actin filament barbed ends play a pivotal role in motile processes. While formins enhance filament assembly, capping protein (CP) blocks polymerization. On their own, they both bind barbed ends with high affinity and very slow dissociation. Their barbed-end binding is thought to be mutually exclusive. CP has recently been shown to be present in filopodia and controls their morphology and dynamics. Here we explore how CP and formins may functionally coregulate filament barbed-end assembly. We show, using kinetic analysis of individual filaments by microfluidics-assisted fluorescence microscopy, that CP and mDia1 formin are able to simultaneously bind barbed ends. This is further confirmed using single-molecule imaging. Their mutually weakened binding enables rapid displacement of one by the other. We show that formin FMNL2 behaves similarly, thus suggesting that this is a general property of formins. Implications in filopodia regulation and barbed-end structural regulation are discussed.

Date: 2015
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/ncomms9730 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9730

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms9730

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().