Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture

Agla J. Fridriksdottir, Jiyoung Kim, René Villadsen, Marie Christine Klitgaard, Branden M. Hopkinson, Ole William Petersen and Lone Rønnov-Jessen ()
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Agla J. Fridriksdottir: Faculty of Health Sciences, University of Copenhagen
Jiyoung Kim: Faculty of Health Sciences, University of Copenhagen
René Villadsen: Faculty of Health Sciences, University of Copenhagen
Marie Christine Klitgaard: Faculty of Health Sciences, University of Copenhagen
Branden M. Hopkinson: Faculty of Health Sciences, University of Copenhagen
Ole William Petersen: Faculty of Health Sciences, University of Copenhagen
Lone Rønnov-Jessen: University of Copenhagen

Nature Communications, 2015, vol. 6, issue 1, 1-12

Abstract: Abstract Investigating the susceptibility of oestrogen receptor-positive (ERpos) normal human breast epithelial cells (HBECs) for clinical purposes or basic research awaits a proficient cell-based assay. Here we set out to identify markers for isolating ERpos cells and to expand what appear to be post-mitotic primary cells into exponentially growing cultures. We report a robust technique for isolating ERpos HBECs from reduction mammoplasties by FACS using two cell surface markers, CD166 and CD117, and an intracellular cytokeratin marker, Ks20.8, for further tracking single cells in culture. We show that ERpos HBECs are released from growth restraint by small molecule inhibitors of TGFβ signalling, and that growth is augmented further in response to oestrogen. Importantly, ER signalling is functionally active in ERpos cells in extended culture. These findings open a new avenue of experimentation with normal ERpos HBECs and provide a basis for understanding the evolution of human breast cancer.

Date: 2015
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DOI: 10.1038/ncomms9786

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