Spindle assembly checkpoint inactivation fails to suppress neuroblast tumour formation in aurA mutant Drosophila

Renaud Caous, Aude Pascal, Pierre Romé, Laurent Richard-Parpaillon, Roger Karess and Régis Giet ()
Additional contact information
Renaud Caous: Institut de Génétique et Développement de Rennes-Université de Rennes I-CNRS- UMR 6290
Aude Pascal: Institut de Génétique et Développement de Rennes-Université de Rennes I-CNRS- UMR 6290
Pierre Romé: Institut de Génétique et Développement de Rennes-Université de Rennes I-CNRS- UMR 6290
Laurent Richard-Parpaillon: Institut de Génétique et Développement de Rennes-Université de Rennes I-CNRS- UMR 6290
Roger Karess: Institut Jacques Monod-Université Paris Diderot-Paris 7
Régis Giet: Institut de Génétique et Développement de Rennes-Université de Rennes I-CNRS- UMR 6290

Nature Communications, 2015, vol. 6, issue 1, 1-9

Abstract: Abstract Tissue homeostasis requires accurate control of cell proliferation, differentiation and chromosome segregation. Drosophila sas-4 and aurA mutants present brain tumours with extra neuroblasts (NBs), defective mitotic spindle assembly and delayed mitosis due to activation of the spindle assembly checkpoint (SAC). Here we inactivate the SAC in aurA and sas-4 mutants to determine whether the generation of aneuploidy compromises NB proliferation. Inactivation of the SAC in the sas-4 mutant impairs NB proliferation and disrupts euploidy. By contrast, disrupting the SAC in the aurA mutant does not prevent NB amplification, tumour formation or chromosome segregation. The monitoring of Mad2 and cyclin B dynamics in live aurA NBs reveals that SAC satisfaction is not coupled to cyclin B degradation. Thus, the NBs of aurA mutants present delayed mitosis, with accurate chromosome segregation occurring in a SAC-independent manner. We report here the existence of an Aurora A-dependent mechanism promoting efficient, timed cyclin B degradation.

Date: 2015
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms9879 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9879

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms9879

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().