Identification and characterization of multiple rubisco activases in chemoautotrophic bacteria

Yi-Chin Candace Tsai, Maria Claribel Lapina, Shashi Bhushan and Oliver Mueller-Cajar ()
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Yi-Chin Candace Tsai: School of Biological Sciences, Nanyang Technological University
Maria Claribel Lapina: School of Biological Sciences, Nanyang Technological University
Shashi Bhushan: School of Biological Sciences, Nanyang Technological University
Oliver Mueller-Cajar: School of Biological Sciences, Nanyang Technological University

Nature Communications, 2015, vol. 6, issue 1, 1-10

Abstract: Abstract Ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) is responsible for almost all biological CO2 assimilation, but forms inhibited complexes with its substrate ribulose-1,5-bisphosphate (RuBP) and other sugar phosphates. The distantly related AAA+ proteins rubisco activase and CbbX remodel inhibited rubisco complexes to effect inhibitor release in plants and α-proteobacteria, respectively. Here we characterize a third class of rubisco activase in the chemolithoautotroph Acidithiobacillus ferrooxidans. Two sets of isoforms of CbbQ and CbbO form hetero-oligomers that function as specific activases for two structurally diverse rubisco forms. Mutational analysis supports a model wherein the AAA+ protein CbbQ functions as motor and CbbO is a substrate adaptor that binds rubisco via a von Willebrand factor A domain. Understanding the mechanisms employed by nature to overcome rubisco’s shortcomings will increase our toolbox for engineering photosynthetic carbon dioxide fixation.

Date: 2015
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DOI: 10.1038/ncomms9883

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