Single-cell analysis and stochastic modelling unveil large cell-to-cell variability in influenza A virus infection

Frank S. Heldt, Sascha Y. Kupke, Sebastian Dorl, Udo Reichl and Timo Frensing ()
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Frank S. Heldt: Max Planck Institute for Dynamics of Complex Technical Systems
Sascha Y. Kupke: Max Planck Institute for Dynamics of Complex Technical Systems
Sebastian Dorl: Max Planck Institute for Dynamics of Complex Technical Systems
Udo Reichl: Max Planck Institute for Dynamics of Complex Technical Systems
Timo Frensing: Max Planck Institute for Dynamics of Complex Technical Systems

Nature Communications, 2015, vol. 6, issue 1, 1-12

Abstract: Abstract Biochemical reactions are subject to stochastic fluctuations that can give rise to cell-to-cell variability. Yet, how this variability affects viral infections, which themselves involve noisy reactions, remains largely elusive. Here we present single-cell experiments and stochastic simulations that reveal a large heterogeneity between influenza A virus (IAV)-infected cells. In particular, experimental data show that progeny virus titres range from 1 to 970 plaque-forming units and intracellular viral RNA (vRNA) levels span three orders of magnitude. Moreover, the segmentation of IAV genomes seems to increase the susceptibility of their replication to noise, since the level of different genome segments can vary substantially within a cell. In addition, simulations suggest that the abortion of virus entry and random degradation of vRNAs can result in a large fraction of non-productive cells after single-hit infection. These results challenge current beliefs that cell population measurements and deterministic simulations are an accurate representation of viral infections.

Date: 2015
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DOI: 10.1038/ncomms9938

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