Axotomy-induced HIF-serotonin signalling axis promotes axon regeneration in C. elegans

Tanimul Alam, Hiroki Maruyama, Chun Li, Strahil Iv. Pastuhov, Paola Nix, Michael Bastiani, Naoki Hisamoto () and Kunihiro Matsumoto ()
Additional contact information
Tanimul Alam: Graduate School of Science, Nagoya University, Chikusa-ku
Hiroki Maruyama: Graduate School of Science, Nagoya University, Chikusa-ku
Chun Li: Graduate School of Science, Nagoya University, Chikusa-ku
Strahil Iv. Pastuhov: Graduate School of Science, Nagoya University, Chikusa-ku
Paola Nix: University of Utah
Michael Bastiani: University of Utah
Naoki Hisamoto: Graduate School of Science, Nagoya University, Chikusa-ku
Kunihiro Matsumoto: Graduate School of Science, Nagoya University, Chikusa-ku

Nature Communications, 2016, vol. 7, issue 1, 1-11

Abstract: Abstract The molecular mechanisms underlying the ability of axons to regenerate after injury remain poorly understood. Here we show that in Caenorhabditis elegans, axotomy induces ectopic expression of serotonin (5-HT) in axotomized non-serotonergic neurons via HIF-1, a hypoxia-inducible transcription factor, and that 5-HT subsequently promotes axon regeneration by autocrine signalling through the SER-7 5-HT receptor. Furthermore, we identify the rhgf-1 and rga-5 genes, encoding homologues of RhoGEF and RhoGAP, respectively, as regulators of axon regeneration. We demonstrate that one pathway initiated by SER-7 acts upstream of the C. elegans RhoA homolog RHO-1 in neuron regeneration, which functions via G12α and RHGF-1. In this pathway, RHO-1 inhibits diacylglycerol kinase, resulting in an increase in diacylglycerol. SER-7 also promotes axon regeneration by activating the cyclic AMP (cAMP) signalling pathway. Thus, HIF-1-mediated activation of 5-HT signalling promotes axon regeneration by activating both the RhoA and cAMP pathways.

Date: 2016
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms10388 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10388

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms10388

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().