Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing

Sanki Tashiro, Tetsuya Handa, Atsushi Matsuda, Takuto Ban, Toru Takigawa, Kazumi Miyasato, Kojiro Ishii, Kazuto Kugou, Kunihiro Ohta, Yasushi Hiraoka, Hisao Masukata and Junko Kanoh ()
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Sanki Tashiro: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan
Tetsuya Handa: Graduate School of Science, Osaka University
Atsushi Matsuda: Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology
Takuto Ban: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan
Toru Takigawa: Graduate School of Science, Osaka University
Kazumi Miyasato: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan
Kojiro Ishii: Graduate School of Frontier Biosciences, Osaka University
Kazuto Kugou: The University of Tokyo
Kunihiro Ohta: The University of Tokyo
Yasushi Hiraoka: Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology
Hisao Masukata: Graduate School of Science, Osaka University
Junko Kanoh: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan

Nature Communications, 2016, vol. 7, issue 1, 1-12

Abstract: Abstract A chromosome is composed of structurally and functionally distinct domains. However, the molecular mechanisms underlying the formation of chromatin structure and the function of subtelomeres, the telomere-adjacent regions, remain obscure. Here we report the roles of the conserved centromeric protein Shugoshin 2 (Sgo2) in defining chromatin structure and functions of the subtelomeres in the fission yeast Schizosaccharomyces pombe. We show that Sgo2 localizes at the subtelomeres preferentially during G2 phase and is essential for the formation of a highly condensed subtelomeric chromatin body ‘knob’. Furthermore, the absence of Sgo2 leads to the derepression of the subtelomeric genes and premature DNA replication at the subtelomeric late origins. Thus, the subtelomeric specialized chromatin domain organized by Sgo2 represses both transcription and replication to ensure proper gene expression and replication timing.

Date: 2016
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DOI: 10.1038/ncomms10393

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