Non-CG DNA methylation is a biomarker for assessing endodermal differentiation capacity in pluripotent stem cells

Lee M. Butcher, Mitsuteru Ito, Minodora Brimpari, Tiffany J. Morris, Filipa A. C. Soares, Lars Ährlund-Richter, Nessa Carey, Ludovic Vallier (), Anne C. Ferguson-Smith () and Stephan Beck ()
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Lee M. Butcher: UCL Cancer Institute, University College London, 72 Huntley Street, London WC1E 6BT, UK
Mitsuteru Ito: University of Cambridge
Minodora Brimpari: Anne McLaren Laboratory, Wellcome Trust and Medical Research Council Stem Cell Institute, University of Cambridge
Tiffany J. Morris: UCL Cancer Institute, University College London, 72 Huntley Street, London WC1E 6BT, UK
Filipa A. C. Soares: Anne McLaren Laboratory, Wellcome Trust and Medical Research Council Stem Cell Institute, University of Cambridge
Lars Ährlund-Richter: Karolinska Institutet
Nessa Carey: PraxisUnico, The Jeffreys Building, St John’s Innovation Park
Ludovic Vallier: Anne McLaren Laboratory, Wellcome Trust and Medical Research Council Stem Cell Institute, University of Cambridge
Anne C. Ferguson-Smith: University of Cambridge
Stephan Beck: UCL Cancer Institute, University College London, 72 Huntley Street, London WC1E 6BT, UK

Nature Communications, 2016, vol. 7, issue 1, 1-8

Abstract: Abstract Non-CG methylation is an unexplored epigenetic hallmark of pluripotent stem cells. Here we report that a reduction in non-CG methylation is associated with impaired differentiation capacity into endodermal lineages. Genome-wide analysis of 2,670 non-CG sites in a discovery cohort of 25 phenotyped human induced pluripotent stem cell (hiPSC) lines revealed unidirectional loss (Δβ=13%, P

Date: 2016
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DOI: 10.1038/ncomms10458

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