RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency

Jun Song, Dongshan Yang, Jie Xu, Tianqing Zhu, Y. Eugene Chen () and Jifeng Zhang ()
Additional contact information
Jun Song: Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center
Dongshan Yang: Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center
Jie Xu: Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center
Tianqing Zhu: Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center
Y. Eugene Chen: Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center
Jifeng Zhang: Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center

Nature Communications, 2016, vol. 7, issue 1, 1-7

Abstract: Abstract Zinc-finger nuclease, transcription activator-like effector nuclease and CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) are becoming major tools for genome editing. Importantly, knock-in in several non-rodent species has been finally achieved thanks to these customizable nucleases; yet the rates remain to be further improved. We hypothesize that inhibiting non-homologous end joining (NHEJ) or enhancing homology-directed repair (HDR) will improve the nuclease-mediated knock-in efficiency. Here we show that the in vitro application of an HDR enhancer, RS-1, increases the knock-in efficiency by two- to five-fold at different loci, whereas NHEJ inhibitor SCR7 has minimal effects. We then apply RS-1 for animal production and have achieved multifold improvement on the knock-in rates as well. Our work presents tools to nuclease-mediated knock-in animal production, and sheds light on improving gene-targeting efficiencies on pluripotent stem cells.

Date: 2016
References: Add references at CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.nature.com/articles/ncomms10548 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10548

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms10548

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().