Optogenetic control of nuclear protein export

Dominik Niopek, Pierre Wehler, Julia Roensch, Roland Eils () and Barbara Di Ventura ()
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Dominik Niopek: German Cancer Research Center (DKFZ)
Pierre Wehler: German Cancer Research Center (DKFZ)
Julia Roensch: German Cancer Research Center (DKFZ)
Roland Eils: German Cancer Research Center (DKFZ)
Barbara Di Ventura: Synthetic Biology Group, Institute for Pharmacy and Biotechnology (IPMB), University of Heidelberg

Nature Communications, 2016, vol. 7, issue 1, 1-9

Abstract: Abstract Active nucleocytoplasmic transport is a key mechanism underlying protein regulation in eukaryotes. While nuclear protein import can be controlled in space and time with a portfolio of optogenetic tools, protein export has not been tackled so far. Here we present a light-inducible nuclear export system (LEXY) based on a single, genetically encoded tag, which enables precise spatiotemporal control over the export of tagged proteins. A constitutively nuclear, chromatin-anchored LEXY variant expands the method towards light inhibition of endogenous protein export by sequestering cellular CRM1 receptors. We showcase the utility of LEXY for cell biology applications by regulating a synthetic repressor as well as human p53 transcriptional activity with light. LEXY is a powerful addition to the optogenetic toolbox, allowing various novel applications in synthetic and cell biology.

Date: 2016
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DOI: 10.1038/ncomms10624

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