Lysosome triggered near-infrared fluorescence imaging of cellular trafficking processes in real time

Marco Grossi, Marina Morgunova, Shane Cheung, Dimitri Scholz, Emer Conroy, Marta Terrile, Angela Panarella, Jeremy C. Simpson, William M. Gallagher and Donal F. O’Shea ()
Additional contact information
Marco Grossi: Royal College of Surgeons in Ireland
Marina Morgunova: Royal College of Surgeons in Ireland
Shane Cheung: Royal College of Surgeons in Ireland
Dimitri Scholz: School of Biomolecular and Biomedical Science, Conway Institute of Biomolecular and Biomedical Research, University College Dublin
Emer Conroy: School of Biomolecular and Biomedical Science, Conway Institute of Biomolecular and Biomedical Research, University College Dublin
Marta Terrile: School of Biomolecular and Biomedical Science, Conway Institute of Biomolecular and Biomedical Research, University College Dublin
Angela Panarella: School of Biology and Environmental Science, Conway Institute of Biomolecular and Biomedical Research, University College Dublin
Jeremy C. Simpson: School of Biology and Environmental Science, Conway Institute of Biomolecular and Biomedical Research, University College Dublin
William M. Gallagher: School of Biomolecular and Biomedical Science, Conway Institute of Biomolecular and Biomedical Research, University College Dublin
Donal F. O’Shea: Royal College of Surgeons in Ireland

Nature Communications, 2016, vol. 7, issue 1, 1-13

Abstract: Abstract Bioresponsive NIR-fluorophores offer the possibility for continual visualization of dynamic cellular processes with added potential for direct translation to in vivo imaging. Here we show the design, synthesis and lysosome-responsive emission properties of a new NIR fluorophore. The NIR fluorescent probe design differs from typical amine functionalized lysosomotropic stains with off/on fluorescence switching controlled by a reversible phenol/phenolate interconversion. Emission from the probe is shown to be highly selective for the lysosomes in co-imaging experiments using a HeLa cell line expressing the lysosomal-associated membrane protein 1 fused to green fluorescent protein. The responsive probe is capable of real-time continuous imaging of fundamental cellular processes such as endocytosis, lysosomal trafficking and efflux in 3D and 4D. The advantage of the NIR emission allows for direct translation to in vivo tumour imaging, which is successfully demonstrated using an MDA-MB-231 subcutaneous tumour model. This bioresponsive NIR fluorophore offers significant potential for use in live cellular and in vivo imaging, for which currently there is a deficit of suitable molecular fluorescent tools.

Date: 2016
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms10855 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10855

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms10855

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().