Actin nucleation at the centrosome controls lymphocyte polarity

Dorian Obino, Francesca Farina, Odile Malbec, Pablo J. Sáez, Mathieu Maurin, Jérémie Gaillard, Florent Dingli, Damarys Loew, Alexis Gautreau, Maria-Isabel Yuseff, Laurent Blanchoin, Manuel Théry and Ana-Maria Lennon-Duménil ()
Additional contact information
Dorian Obino: INSERM—U932 Immunité et Cancer, Institut Curie, PSL Research University
Francesca Farina: CytoMorpho Lab, Biosciences & Biotechnology Institute of Grenoble, UMR5168, CEA/INRA/CNRS/Université Grenoble-Alpes
Odile Malbec: INSERM—U932 Immunité et Cancer, Institut Curie, PSL Research University
Pablo J. Sáez: INSERM—U932 Immunité et Cancer, Institut Curie, PSL Research University
Mathieu Maurin: INSERM—U932 Immunité et Cancer, Institut Curie, PSL Research University
Jérémie Gaillard: CytoMorpho Lab, Biosciences & Biotechnology Institute of Grenoble, UMR5168, CEA/INRA/CNRS/Université Grenoble-Alpes
Florent Dingli: Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie, PSL Research University
Damarys Loew: Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie, PSL Research University
Alexis Gautreau: Ecole Polytechnique, CNRS UMR7654
Maria-Isabel Yuseff: Pontificia Universidad Catolica de Chile
Laurent Blanchoin: CytoMorpho Lab, Biosciences & Biotechnology Institute of Grenoble, UMR5168, CEA/INRA/CNRS/Université Grenoble-Alpes
Manuel Théry: CytoMorpho Lab, Biosciences & Biotechnology Institute of Grenoble, UMR5168, CEA/INRA/CNRS/Université Grenoble-Alpes
Ana-Maria Lennon-Duménil: INSERM—U932 Immunité et Cancer, Institut Curie, PSL Research University

Nature Communications, 2016, vol. 7, issue 1, 1-14

Abstract: Abstract Cell polarity is required for the functional specialization of many cell types including lymphocytes. A hallmark of cell polarity is the reorientation of the centrosome that allows repositioning of organelles and vesicles in an asymmetric fashion. The mechanisms underlying centrosome polarization are not fully understood. Here we found that in resting lymphocytes, centrosome-associated Arp2/3 locally nucleates F-actin, which is needed for centrosome tethering to the nucleus via the LINC complex. Upon lymphocyte activation, Arp2/3 is partially depleted from the centrosome as a result of its recruitment to the immune synapse. This leads to a reduction in F-actin nucleation at the centrosome and thereby allows its detachment from the nucleus and polarization to the synapse. Therefore, F-actin nucleation at the centrosome—regulated by the availability of the Arp2/3 complex—determines its capacity to polarize in response to external stimuli.

Date: 2016
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DOI: 10.1038/ncomms10969

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