Neogenin recruitment of the WAVE regulatory complex maintains adherens junction stability and tension

Natalie K. Lee, Ka Wai Fok, Amanda White, Nicole H. Wilson, Conor J. O’Leary, Hayley L. Cox, Magdalene Michael, Alpha S. Yap and Helen M. Cooper ()
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Natalie K. Lee: Queensland Brain Institute, The University of Queensland
Ka Wai Fok: Queensland Brain Institute, The University of Queensland
Amanda White: Queensland Brain Institute, The University of Queensland
Nicole H. Wilson: Queensland Brain Institute, The University of Queensland
Conor J. O’Leary: Queensland Brain Institute, The University of Queensland
Hayley L. Cox: Queensland Brain Institute, The University of Queensland
Magdalene Michael: Institute for Molecular Bioscience, The University of Queensland
Alpha S. Yap: Institute for Molecular Bioscience, The University of Queensland
Helen M. Cooper: Queensland Brain Institute, The University of Queensland

Nature Communications, 2016, vol. 7, issue 1, 1-13

Abstract: Abstract To maintain tissue integrity during epithelial morphogenesis, adherens junctions (AJs) must resist the mechanical stresses exerted by dynamic tissue movements. Junctional stability is dependent on actomyosin contractility within the actin ring. Here we describe a novel function for the axon guidance receptor, Neogenin, as a key component of the actin nucleation machinery governing junctional stability. Loss of Neogenin perturbs AJs and attenuates junctional tension. Neogenin promotes actin nucleation at AJs by recruiting the Wave regulatory complex (WRC) and Arp2/3. A direct interaction between the Neogenin WIRS domain and the WRC is crucial for the spatially restricted recruitment of the WRC to the junction. Thus, we provide the first example of a functional WIRS–WRC interaction in epithelia. We further show that Neogenin regulates cadherin recycling at the AJ. In summary, we identify Neogenin as a pivotal component of the AJ, where it influences both cadherin dynamics and junctional tension.

Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11082

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DOI: 10.1038/ncomms11082

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