Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy

Qinyi Fu, Benjamin L. Martin, David Q. Matus and Liang Gao ()
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Qinyi Fu: Stony Brook University
Benjamin L. Martin: Stony Brook University
David Q. Matus: Stony Brook University
Liang Gao: Stony Brook University

Nature Communications, 2016, vol. 7, issue 1, 1-10

Abstract: Abstract Despite the progress made in selective plane illumination microscopy, high-resolution 3D live imaging of multicellular specimens remains challenging. Tiling light-sheet selective plane illumination microscopy (TLS-SPIM) with real-time light-sheet optimization was developed to respond to the challenge. It improves the 3D imaging ability of SPIM in resolving complex structures and optimizes SPIM live imaging performance by using a real-time adjustable tiling light sheet and creating a flexible compromise between spatial and temporal resolution. We demonstrate the 3D live imaging ability of TLS-SPIM by imaging cellular and subcellular behaviours in live C. elegans and zebrafish embryos, and show how TLS-SPIM can facilitate cell biology research in multicellular specimens by studying left-right symmetry breaking behaviour of C. elegans embryos.

Date: 2016
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DOI: 10.1038/ncomms11088

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