Generation of stem cell-derived β-cells from patients with type 1 diabetes

Jeffrey R. Millman (), Chunhui Xie, Alana Van Dervort, Mads Gürtler, Felicia W. Pagliuca and Douglas A. Melton ()
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Jeffrey R. Millman: Metabolism and Lipid Research, Washington University School of Medicine, Campus Box 8127, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
Chunhui Xie: Harvard Stem Cell Institute, Harvard University
Alana Van Dervort: Case Western Reserve University School of Medicine
Mads Gürtler: Harvard Stem Cell Institute, Harvard University
Felicia W. Pagliuca: Semma Therapeutics, Inc.
Douglas A. Melton: Harvard Stem Cell Institute, Harvard University

Nature Communications, 2016, vol. 7, issue 1, 1-9

Abstract: Abstract We recently reported the scalable in vitro production of functional stem cell-derived β-cells (SC-β cells). Here we extend this approach to generate the first SC-β cells from type 1 diabetic patients (T1D). β-cells are destroyed during T1D disease progression, making it difficult to extensively study them in the past. These T1D SC-β cells express β-cell markers, respond to glucose both in vitro and in vivo, prevent alloxan-induced diabetes in mice and respond to anti-diabetic drugs. Furthermore, we use an in vitro disease model to demonstrate the cells respond to different forms of β-cell stress. Using these assays, we find no major differences in T1D SC-β cells compared with SC-β cells derived from non-diabetic patients. These results show that T1D SC-β cells could potentially be used for the treatment of diabetes, drug screening and the study of β-cell biology.

Date: 2016
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DOI: 10.1038/ncomms11463

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