The Arabidopsis NPF3 protein is a GA transporter

Iris Tal, Yi Zhang, Morten Egevang Jørgensen, Odelia Pisanty, Inês C. R. Barbosa, Melina Zourelidou, Thomas Regnault, Christoph Crocoll, Carl Erik Olsen, Roy Weinstain, Claus Schwechheimer, Barbara Ann Halkier, Hussam Hassan Nour-Eldin, Mark Estelle () and Eilon Shani ()
Additional contact information
Iris Tal: Tel Aviv University
Yi Zhang: Howard Hughes Medical Institute and Section of Cell and Developmental Biology, University of California San Diego
Morten Egevang Jørgensen: DynaMo Center, Faculty of Science, University of Copenhagen
Odelia Pisanty: Tel Aviv University
Inês C. R. Barbosa: Plant Systems Biology, Technische Universität München
Melina Zourelidou: Plant Systems Biology, Technische Universität München
Thomas Regnault: Plant Systems Biology, Technische Universität München
Christoph Crocoll: DynaMo Center, Faculty of Science, University of Copenhagen
Carl Erik Olsen: Faculty of Science, University of Copenhagen
Roy Weinstain: Tel Aviv University
Claus Schwechheimer: Plant Systems Biology, Technische Universität München
Barbara Ann Halkier: DynaMo Center, Faculty of Science, University of Copenhagen
Hussam Hassan Nour-Eldin: DynaMo Center, Faculty of Science, University of Copenhagen
Mark Estelle: Howard Hughes Medical Institute and Section of Cell and Developmental Biology, University of California San Diego
Eilon Shani: Tel Aviv University

Nature Communications, 2016, vol. 7, issue 1, 1-11

Abstract: Abstract Gibberellins (GAs) are plant hormones that promote a wide range of developmental processes. While GA signalling is well understood, little is known about how GA is transported or how GA distribution is regulated. Here we utilize fluorescently labelled GAs (GA-Fl) to screen for Arabidopsis mutants deficient in GA transport. We show that the NPF3 transporter efficiently transports GA across cell membranes in vitro and GA-Fl in vivo. NPF3 is expressed in root endodermis and repressed by GA. NPF3 is targeted to the plasma membrane and subject to rapid BFA-dependent recycling. We show that abscisic acid (ABA), an antagonist of GA, is also transported by NPF3 in vitro. ABA promotes NPF3 expression and GA-Fl uptake in plants. On the basis of these results, we propose that GA distribution and activity in Arabidopsis is partly regulated by NPF3 acting as an influx carrier and that GA–ABA interaction may occur at the level of transport.

Date: 2016
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DOI: 10.1038/ncomms11486

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