A microfluidics-based in vitro model of the gastrointestinal human–microbe interface

Pranjul Shah, Joëlle V. Fritz, Enrico Glaab, Mahesh S. Desai, Kacy Greenhalgh, Audrey Frachet, Magdalena Niegowska, Matthew Estes, Christian Jäger, Carole Seguin-Devaux, Frederic Zenhausern and Paul Wilmes ()
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Pranjul Shah: Luxembourg Centre for Systems Biomedicine, University of Luxembourg
Joëlle V. Fritz: Luxembourg Centre for Systems Biomedicine, University of Luxembourg
Enrico Glaab: Luxembourg Centre for Systems Biomedicine, University of Luxembourg
Mahesh S. Desai: Luxembourg Centre for Systems Biomedicine, University of Luxembourg
Kacy Greenhalgh: Luxembourg Centre for Systems Biomedicine, University of Luxembourg
Audrey Frachet: Luxembourg Centre for Systems Biomedicine, University of Luxembourg
Magdalena Niegowska: Luxembourg Centre for Systems Biomedicine, University of Luxembourg
Matthew Estes: Center for Applied Nanobioscience and Medicine, University of Arizona
Christian Jäger: Luxembourg Centre for Systems Biomedicine, University of Luxembourg
Carole Seguin-Devaux: Luxembourg Institute of Health
Frederic Zenhausern: Center for Applied Nanobioscience and Medicine, University of Arizona
Paul Wilmes: Luxembourg Centre for Systems Biomedicine, University of Luxembourg

Nature Communications, 2016, vol. 7, issue 1, 1-15

Abstract: Abstract Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics-based model (HuMiX, human–microbial crosstalk), which allows co-culture of human and microbial cells under conditions representative of the gastrointestinal human–microbe interface. We demonstrate the ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions. In addition, we show that the co-culture of human epithelial cells with the obligate anaerobe Bacteroides caccae and LGG results in a transcriptional response, which is distinct from that of a co-culture solely comprising LGG. HuMiX facilitates investigations of host–microbe molecular interactions and provides insights into a range of fundamental research questions linking the gastrointestinal microbiome to human health and disease.

Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11535

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DOI: 10.1038/ncomms11535

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