Evidence that phytochrome functions as a protein kinase in plant light signalling

Ah-Young Shin, Yun-Jeong Han, Ayoung Baek, Taeho Ahn, Soo Young Kim, Thai Son Nguyen, Minky Son, Keun Woo Lee, Yu Shen, Pill-Soon Song and Jeong-Il Kim ()
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Ah-Young Shin: Chonnam National University
Yun-Jeong Han: Chonnam National University
Ayoung Baek: Systems and Synthetic Agrobiotech Center (SSAC), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Research Institute of Natural Science (RINS), Gyeongsang National University
Taeho Ahn: College of Veterinary Medicine, Chonnam National University
Soo Young Kim: Chonnam National University
Thai Son Nguyen: Chonnam National University
Minky Son: Systems and Synthetic Agrobiotech Center (SSAC), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Research Institute of Natural Science (RINS), Gyeongsang National University
Keun Woo Lee: Systems and Synthetic Agrobiotech Center (SSAC), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Research Institute of Natural Science (RINS), Gyeongsang National University
Yu Shen: BioVision Inc.
Pill-Soon Song: Faculty of Biotechnology and Subtropical Horticulture Research Institute, Jeju National University
Jeong-Il Kim: Chonnam National University

Nature Communications, 2016, vol. 7, issue 1, 1-13

Abstract: Abstract It has been suggested that plant phytochromes are autophosphorylating serine/threonine kinases. However, the biochemical properties and functional roles of putative phytochrome kinase activity in plant light signalling are largely unknown. Here, we describe the biochemical and functional characterization of Avena sativa phytochrome A (AsphyA) as a potential protein kinase. We provide evidence that phytochrome-interacting factors (PIFs) are phosphorylated by phytochromes in vitro. Domain mapping of AsphyA shows that the photosensory core region consisting of PAS-GAF-PHY domains in the N-terminal is required for the observed kinase activity. Moreover, we demonstrate that transgenic plants expressing mutant versions of AsphyA, which display reduced activity in in vitro kinase assays, show hyposensitive responses to far-red light. Further analysis reveals that far-red light-induced phosphorylation and degradation of PIF3 are significantly reduced in these transgenic plants. Collectively, these results suggest a positive relationship between phytochrome kinase activity and photoresponses in plants.

Date: 2016
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DOI: 10.1038/ncomms11545

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