The fast-recycling receptor Megalin defines the apical recycling pathway of epithelial cells

Andres E. Perez Bay (), Ryan Schreiner, Ignacio Benedicto, Maria Paz Marzolo, Jason Banfelder, Alan M. Weinstein and Enrique J. Rodriguez-Boulan ()
Additional contact information
Andres E. Perez Bay: Margaret Dyson Vision Research Institute, Weill Medical College of Cornell University
Ryan Schreiner: Margaret Dyson Vision Research Institute, Weill Medical College of Cornell University
Ignacio Benedicto: Margaret Dyson Vision Research Institute, Weill Medical College of Cornell University
Maria Paz Marzolo: Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile
Jason Banfelder: Weill Medical College of Cornell University
Alan M. Weinstein: Weill Medical College of Cornell University
Enrique J. Rodriguez-Boulan: Margaret Dyson Vision Research Institute, Weill Medical College of Cornell University

Nature Communications, 2016, vol. 7, issue 1, 1-15

Abstract: Abstract The basolateral recycling and transcytotic pathways of epithelial cells were previously defined using markers such as transferrin (TfR) and polymeric IgA (pIgR) receptors. In contrast, our knowledge of the apical recycling pathway remains fragmentary. Here we utilize quantitative live-imaging and mathematical modelling to outline the recycling pathway of Megalin (LRP-2), an apical receptor with key developmental and renal functions, in MDCK cells. We show that, like TfR, Megalin is a long-lived and fast-recycling receptor. Megalin enters polarized MDCK cells through segregated apical sorting endosomes and subsequently intersects the TfR and pIgR pathways at a perinuclear Rab11-negative compartment termed common recycling endosomes (CRE). Whereas TfR recycles to the basolateral membrane from CRE, Megalin, like pIgR, traffics to subapical Rab11-positive apical recycling endosomes (ARE) and reaches the apical membrane in a microtubule- and Rab11-dependent manner. Hence, Megalin defines the apical recycling pathway of epithelia, with CRE as its apical sorting station.

Date: 2016
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms11550 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11550

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms11550

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().