Live single-cell laser tag

Loïc Binan, Javier Mazzaferri, Karine Choquet, Louis-Etienne Lorenzo, Yu Chang Wang, El Bachir Affar, Yves De Koninck, Jiannis Ragoussis, Claudia L. Kleinman () and Santiago Costantino ()
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Loïc Binan: Research Center of the Maisonneuve-Rosemont Hospital
Javier Mazzaferri: Research Center of the Maisonneuve-Rosemont Hospital
Karine Choquet: McGill University
Louis-Etienne Lorenzo: Institut Universitaire en Santé Mentale de Québec
Yu Chang Wang: McGill University and Genome Quebec Innovation Centre
El Bachir Affar: Research Center of the Maisonneuve-Rosemont Hospital
Yves De Koninck: Institut Universitaire en Santé Mentale de Québec
Jiannis Ragoussis: McGill University
Claudia L. Kleinman: McGill University
Santiago Costantino: Research Center of the Maisonneuve-Rosemont Hospital

Nature Communications, 2016, vol. 7, issue 1, 1-8

Abstract: Abstract The ability to conduct image-based, non-invasive cell tagging, independent of genetic engineering, is key to cell biology applications. Here we introduce cell labelling via photobleaching (CLaP), a method that enables instant, specific tagging of individual cells based on a wide array of criteria such as shape, behaviour or positional information. CLaP uses laser illumination to crosslink biotin onto the plasma membrane, coupled with streptavidin conjugates to label individual cells for genomic, cell-tracking, flow cytometry or ultra-microscopy applications. We show that the incorporated mark is stable, non-toxic, retained for several days, and transferred by cell division but not to adjacent cells in culture. To demonstrate the potential of CLaP for genomic applications, we combine CLaP with microfluidics-based single-cell capture followed by transcriptome-wide next-generation sequencing. Finally, we show that CLaP can also be exploited for inducing transient cell adhesion to substrates for microengineering cultures with spatially patterned cell types.

Date: 2016
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DOI: 10.1038/ncomms11636

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