In vivo imaging of clock gene expression in multiple tissues of freely moving mice

Toshiyuki Hamada, Kenneth Sutherland, Masayori Ishikawa (), Naoki Miyamoto, Sato Honma, Hiroki Shirato and Ken-ichi Honma ()
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Toshiyuki Hamada: Hokkaido University Graduate School of Medicine
Kenneth Sutherland: Hokkaido University Graduate School of Medicine
Masayori Ishikawa: Hokkaido University Graduate School of Medicine
Naoki Miyamoto: Hokkaido University Graduate School of Medicine
Sato Honma: Hokkaido University Graduate School of Medicine
Hiroki Shirato: Global Station for Quantum Medical Science and Engineering, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University
Ken-ichi Honma: Hokkaido University Graduate School of Medicine

Nature Communications, 2016, vol. 7, issue 1, 1-13

Abstract: Abstract Clock genes are expressed throughout the body, although how they oscillate in unrestrained animals is not known. Here, we show an in vivo imaging technique that enables long-term simultaneous imaging of multiple tissues. We use dual-focal 3D tracking and signal-intensity calibration to follow gene expression in a target area. We measure circadian rhythms of clock genes in the olfactory bulb, right and left ears and cortices, and the skin. In addition, the kinetic relationship between gene expression and physiological responses to experimental cues is monitored. Under stable conditions gene expression is in phase in all tissues. In response to a long-duration light pulse, the olfactory bulb shifts faster than other tissues. In Cry1−/− Cry2−/− arrhythmic mice circadian oscillation is absent in all tissues. Thus, our system successfully tracks circadian rhythms in clock genes in multiple tissues in unrestrained mice.

Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11705

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DOI: 10.1038/ncomms11705

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