Aurora-A recruitment and centrosomal maturation are regulated by a Golgi-activated pool of Src during G2

Maria Luisa Barretta, Daniela Spano, Chiara D’Ambrosio, Romina Ines Cervigni, Andrea Scaloni, Daniela Corda and Antonino Colanzi ()
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Maria Luisa Barretta: Institute of Protein Biochemistry (IBP), National Research Council (CNR)
Daniela Spano: Institute of Protein Biochemistry (IBP), National Research Council (CNR)
Chiara D’Ambrosio: Proteomics and Mass Spectrometry Laboratory, Institute for the Animal Production System in the Mediterranean Environment, ISPAAM, National Research Council (CNR)
Romina Ines Cervigni: Institute of Protein Biochemistry (IBP), National Research Council (CNR)
Andrea Scaloni: Proteomics and Mass Spectrometry Laboratory, Institute for the Animal Production System in the Mediterranean Environment, ISPAAM, National Research Council (CNR)
Daniela Corda: Institute of Protein Biochemistry (IBP), National Research Council (CNR)
Antonino Colanzi: Institute of Protein Biochemistry (IBP), National Research Council (CNR)

Nature Communications, 2016, vol. 7, issue 1, 1-13

Abstract: Abstract The Golgi apparatus is composed of stacks of cisternae laterally connected by tubules to form a ribbon-like structure. At the onset of mitosis, the Golgi ribbon is broken down into discrete stacks, which then undergo further fragmentation. This ribbon cleavage is required for G2/M transition, which thus indicates that a ‘Golgi mitotic checkpoint’ couples Golgi inheritance with cell cycle transition. We previously showed that the Golgi-checkpoint regulates the centrosomal recruitment of the mitotic kinase Aurora-A; however, how the Golgi unlinking regulates this recruitment was unknown. Here we show that, in G2, Aurora-A recruitment is promoted by activated Src at the Golgi. Our data provide evidence that Src and Aurora-A interact upon Golgi ribbon fragmentation; Src phosphorylates Aurora-A at tyrosine 148 and this specific phosphorylation is required for Aurora-A localization at the centrosomes. This process, pivotal for centrosome maturation, is a fundamental prerequisite for proper spindle formation and chromosome segregation.

Date: 2016
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DOI: 10.1038/ncomms11727

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