In vivo modulation of endothelial polarization by Apelin receptor signalling

Hyouk-Bum Kwon (), Shengpeng Wang, Christian S. M. Helker, S. Javad Rasouli, Hans-Martin Maischein, Stefan Offermanns, Wiebke Herzog and Didier Y. R. Stainier ()
Additional contact information
Hyouk-Bum Kwon: Max Planck Institute for Heart and Lung Research
Shengpeng Wang: Max Planck Institute for Heart and Lung Research
Christian S. M. Helker: Max Planck Institute for Heart and Lung Research
S. Javad Rasouli: Max Planck Institute for Heart and Lung Research
Hans-Martin Maischein: Max Planck Institute for Heart and Lung Research
Stefan Offermanns: Max Planck Institute for Heart and Lung Research
Wiebke Herzog: University of Münster
Didier Y. R. Stainier: Max Planck Institute for Heart and Lung Research

Nature Communications, 2016, vol. 7, issue 1, 1-12

Abstract: Abstract Endothelial cells (ECs) respond to shear stress by aligning in the direction of flow. However, how ECs respond to flow in complex in vivo environments is less clear. Here we describe an endothelial-specific transgenic zebrafish line, whereby the Golgi apparatus is labelled to allow for in vivo analysis of endothelial polarization. We find that most ECs polarize within 4.5 h after the onset of vigorous blood flow and, by manipulating cardiac function, observe that flow-induced EC polarization is a dynamic and reversible process. Based on its role in EC migration, we analyse the role of Apelin signalling in EC polarization and find that it is critical for this process. Knocking down Apelin receptor function in human primary ECs also affects their polarization. Our study provides new tools to analyse the mechanisms of EC polarization in vivo and reveals an important role in this process for a signalling pathway implicated in cardiovascular disease.

Date: 2016
References: Add references at CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.nature.com/articles/ncomms11805 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11805

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms11805

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().