High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material

Felix F. Loeffler (), Tobias C. Foertsch, Roman Popov, Daniela S. Mattes, Martin Schlageter, Martyna Sedlmayr, Barbara Ridder, Florian-Xuan Dang, Clemens von Bojničić-Kninski, Laura K. Weber, Andrea Fischer, Juliane Greifenstein, Valentina Bykovskaya, Ivan Buliev, F. Ralf Bischoff, Lothar Hahn, Michael A. R. Meier, Stefan Bräse, Annie K. Powell, Teodor Silviu Balaban, Frank Breitling () and Alexander Nesterov-Mueller ()
Additional contact information
Felix F. Loeffler: Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT)
Tobias C. Foertsch: Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT)
Roman Popov: Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT)
Daniela S. Mattes: Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT)
Martin Schlageter: Karlsruhe Institute of Technology, Institute for Inorganic Chemistry
Martyna Sedlmayr: Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT)
Barbara Ridder: Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT)
Clemens von Bojničić-Kninski: Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT)
Laura K. Weber: Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT)
Andrea Fischer: Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT)
Juliane Greifenstein: Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT)
Valentina Bykovskaya: Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT)
Ivan Buliev: Technical University of Varna
F. Ralf Bischoff: German Cancer Research Center
Lothar Hahn: Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT)
Stefan Bräse: Karlsruhe Institute of Technology, Institute of Toxicology and Genetics (ITG)
Annie K. Powell: Karlsruhe Institute of Technology, Institute for Inorganic Chemistry
Frank Breitling: Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT)
Alexander Nesterov-Mueller: Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT)

Nature Communications, 2016, vol. 7, issue 1, 1-9

Abstract: Abstract Laser writing is used to structure surfaces in many different ways in materials and life sciences. However, combinatorial patterning applications are still limited. Here we present a method for cost-efficient combinatorial synthesis of very-high-density peptide arrays with natural and synthetic monomers. A laser automatically transfers nanometre-thin solid material spots from different donor slides to an acceptor. Each donor bears a thin polymer film, embedding one type of monomer. Coupling occurs in a separate heating step, where the matrix becomes viscous and building blocks diffuse and couple to the acceptor surface. Furthermore, we can consecutively deposit two material layers of activation reagents and amino acids. Subsequent heat-induced mixing facilitates an in situ activation and coupling of the monomers. This allows us to incorporate building blocks with click chemistry compatibility or a large variety of commercially available non-activated, for example, posttranslationally modified building blocks into the array’s peptides with >17,000 spots per cm2.

Date: 2016
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DOI: 10.1038/ncomms11844

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