The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction

Catherine A. Wakeman, Jessica L. Moore, Michael J. Noto, Yaofang Zhang, Marc D. Singleton, Boone M. Prentice, Benjamin A. Gilston, Ryan S. Doster, Jennifer A. Gaddy, Walter J. Chazin, Richard M. Caprioli () and Eric P. Skaar ()
Additional contact information
Catherine A. Wakeman: Tennessee Valley Healthcare Systems
Jessica L. Moore: Vanderbilt University
Michael J. Noto: Vanderbilt University School of Medicine
Yaofang Zhang: Vanderbilt University School of Medicine
Marc D. Singleton: Vanderbilt University
Boone M. Prentice: Vanderbilt University School of Medicine
Benjamin A. Gilston: Vanderbilt University School of Medicine
Ryan S. Doster: Vanderbilt University School of Medicine
Jennifer A. Gaddy: Tennessee Valley Healthcare Systems
Walter J. Chazin: Vanderbilt University
Richard M. Caprioli: Vanderbilt University
Eric P. Skaar: Tennessee Valley Healthcare Systems

Nature Communications, 2016, vol. 7, issue 1, 1-12

Abstract: Abstract Microorganisms form biofilms containing differentiated cell populations. To determine factors driving differentiation, we herein visualize protein and metal distributions within Pseudomonas aeruginosa biofilms using imaging mass spectrometry. These in vitro experiments reveal correlations between differential protein distribution and metal abundance. Notably, zinc- and manganese-depleted portions of the biofilm repress the production of anti-staphylococcal molecules. Exposure to calprotectin (a host protein known to sequester metal ions at infectious foci) recapitulates responses occurring within metal-deplete portions of the biofilm and promotes interaction between P. aeruginosa and Staphylococcus aureus. Consistent with these results, the presence of calprotectin promotes co-colonization of the murine lung, and polymicrobial communities are found to co-exist in calprotectin-enriched airspaces of a cystic fibrosis lung explant. These findings, which demonstrate that metal fluctuations are a driving force of microbial community structure, have clinical implications because of the frequent occurrence of P. aeruginosa and S. aureus co-infections.

Date: 2016
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms11951 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11951

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms11951

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().