The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

Carsten Rautengarten, Berit Ebert, Lifeng Liu, Solomon Stonebloom, Andreia M. Smith-Moritz, Markus Pauly, Ariel Orellana, Henrik Vibe Scheller and Joshua L. Heazlewood ()
Additional contact information
Carsten Rautengarten: Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA
Berit Ebert: Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA
Lifeng Liu: University of California
Solomon Stonebloom: Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA
Andreia M. Smith-Moritz: Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA
Markus Pauly: University of California
Ariel Orellana: Centro de Biotecnología Vegetal, Facultad de Ciencias Biológicas, Universidad Andrés Bello
Henrik Vibe Scheller: Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA
Joshua L. Heazlewood: Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA

Nature Communications, 2016, vol. 7, issue 1, 1-10

Abstract: Abstract Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.

Date: 2016
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms12119 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12119

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms12119

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().