Global changes of the RNA-bound proteome during the maternal-to-zygotic transition in Drosophila

Vasiliy O. Sysoev, Bernd Fischer, Christian K. Frese, Ishaan Gupta, Jeroen Krijgsveld, Matthias W. Hentze, Alfredo Castello and Anne Ephrussi ()
Additional contact information
Vasiliy O. Sysoev: European Molecular Biology Laboratory
Bernd Fischer: European Molecular Biology Laboratory
Christian K. Frese: European Molecular Biology Laboratory
Ishaan Gupta: European Molecular Biology Laboratory
Jeroen Krijgsveld: European Molecular Biology Laboratory
Matthias W. Hentze: European Molecular Biology Laboratory
Alfredo Castello: European Molecular Biology Laboratory
Anne Ephrussi: European Molecular Biology Laboratory

Nature Communications, 2016, vol. 7, issue 1, 1-11

Abstract: Abstract The maternal-to-zygotic transition (MZT) is a process that occurs in animal embryos at the earliest developmental stages, during which maternally deposited mRNAs and other molecules are degraded and replaced by products of the zygotic genome. The zygotic genome is not activated immediately upon fertilization, and in the pre-MZT embryo post-transcriptional control by RNA-binding proteins (RBPs) orchestrates the first steps of development. To identify relevant Drosophila RBPs organism-wide, we refined the RNA interactome capture method for comparative analysis of the pre- and post-MZT embryos. We determine 523 proteins as high-confidence RBPs, half of which were not previously reported to bind RNA. Comparison of the RNA interactomes of pre- and post-MZT embryos reveals high dynamicity of the RNA-bound proteome during early development, and suggests active regulation of RNA binding of some RBPs. This resource provides unprecedented insight into the system of RBPs that govern the earliest steps of Drosophila development.

Date: 2016
References: Add references at CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.nature.com/articles/ncomms12128 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12128

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms12128

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().