Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture

Sara Pijuan-Galitó (), Christoffer Tamm, Jens Schuster, Maria Sobol, Lars Forsberg, Catherine L. R. Merry and Cecilia Annerén ()
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Sara Pijuan-Galitó: Box 582, Uppsala University
Christoffer Tamm: Box 582, Uppsala University
Jens Schuster: Genetics and Pathology and Science for Life Laboratory
Maria Sobol: Genetics and Pathology and Science for Life Laboratory
Lars Forsberg: Genetics and Pathology and Science for Life Laboratory
Catherine L. R. Merry: Box 582, Uppsala University
Cecilia Annerén: Box 582, Uppsala University

Nature Communications, 2016, vol. 7, issue 1, 1-12

Abstract: Abstract Reliable, scalable and time-efficient culture methods are required to fully realize the clinical and industrial applications of human pluripotent stem (hPS) cells. Here we present a completely defined, xeno-free medium that supports long-term propagation of hPS cells on uncoated tissue culture plastic. The medium consists of the Essential 8 (E8) formulation supplemented with inter-α-inhibitor (IαI), a human serum-derived protein, recently demonstrated to activate key pluripotency pathways in mouse PS cells. IαI efficiently induces attachment and long-term growth of both embryonic and induced hPS cell lines when added as a soluble protein to the medium at seeding. IαI supplementation efficiently supports adaptation of feeder-dependent hPS cells to xeno-free conditions, clonal growth as well as single-cell survival in the absence of Rho-associated kinase inhibitor (ROCKi). This time-efficient and simplified culture method paves the way for large-scale, high-throughput hPS cell culture, and will be valuable for both basic research and commercial applications.

Date: 2016
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DOI: 10.1038/ncomms12170

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