Target-dependent biogenesis of cognate microRNAs in human cells

Mainak Bose and Suvendra N. Bhattacharyya ()
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Mainak Bose: RNA Biology Research Laboratory, CSIR-Indian Institute of Chemical Biology, 4, Raja SC Mullick Road, Kolkata 700032, India
Suvendra N. Bhattacharyya: RNA Biology Research Laboratory, CSIR-Indian Institute of Chemical Biology, 4, Raja SC Mullick Road, Kolkata 700032, India

Nature Communications, 2016, vol. 7, issue 1, 1-13

Abstract: Abstract Extensive research has established how miRNAs regulate target mRNAs by translation repression and/or endonucleolytic degradation in metazoans. However, information related to the effect of target mRNA on biogenesis and stability of corresponding miRNAs in animals is limited. Here we report regulated biogenesis of cognate miRNAs by their target mRNAs. Enhanced pre-miRNA processing by AGO-associated DICER1 contributes to this increased miRNP formation. The processed miRNAs are loaded onto AGO2 to form functionally competent miRISCs both in vivo and also in a cell-free in vitro system. Thus, we identify an additional layer of posttranscriptional regulation that helps the cell to maintain requisite levels of mature forms of respective miRNAs by modulating their processing in a target-dependent manner, a process happening for miR-122 during stress reversal in human hepatic cells.

Date: 2016
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DOI: 10.1038/ncomms12200

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