Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care

Pablo Perez-Pinera, Ningren Han, Sara Cleto, Jicong Cao, Oliver Purcell, Kartik A. Shah, Kevin Lee, Rajeev Ram and Timothy K. Lu ()
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Pablo Perez-Pinera: Synthetic Biology Group, Massachusetts Institute of Technology
Ningren Han: Research Laboratory of Electronics, Massachusetts Institute of Technology
Sara Cleto: Synthetic Biology Group, Massachusetts Institute of Technology
Jicong Cao: Synthetic Biology Group, Massachusetts Institute of Technology
Oliver Purcell: Synthetic Biology Group, Massachusetts Institute of Technology
Kartik A. Shah: The David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Kevin Lee: Pharyx Inc.
Rajeev Ram: Research Laboratory of Electronics, Massachusetts Institute of Technology
Timothy K. Lu: Synthetic Biology Group, Massachusetts Institute of Technology

Nature Communications, 2016, vol. 7, issue 1, 1-10

Abstract: Abstract Current biopharmaceutical manufacturing systems are not compatible with portable or distributed production of biologics, as they typically require the development of single biologic-producing cell lines followed by their cultivation at very large scales. Therefore, it remains challenging to treat patients in short time frames, especially in remote locations with limited infrastructure. To overcome these barriers, we developed a platform using genetically engineered Pichia pastoris strains designed to secrete multiple proteins on programmable cues in an integrated, benchtop, millilitre-scale microfluidic device. We use this platform for rapid and switchable production of two biologics from a single yeast strain as specified by the operator. Our results demonstrate selectable and near-single-dose production of these biologics in

Date: 2016
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DOI: 10.1038/ncomms12211

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