Super-resolution spectroscopic microscopy via photon localization

Biqin Dong, Luay Almassalha, Ben E. Urban, The-Quyen Nguyen, Satya Khuon, Teng-Leong Chew, Vadim Backman, Cheng Sun and Hao F. Zhang ()
Additional contact information
Biqin Dong: Northwestern University
Luay Almassalha: Northwestern University
Ben E. Urban: Northwestern University
The-Quyen Nguyen: Northwestern University
Satya Khuon: Advanced Imaging Center, Howard Hughes Medical Institute
Teng-Leong Chew: Advanced Imaging Center, Howard Hughes Medical Institute
Vadim Backman: Northwestern University
Cheng Sun: Northwestern University
Hao F. Zhang: Northwestern University

Nature Communications, 2016, vol. 7, issue 1, 1-8

Abstract: Abstract Traditional photon localization microscopy analyses only the spatial distributions of photons emitted by individual molecules to reconstruct super-resolution optical images. Unfortunately, however, the highly valuable spectroscopic information from these photons have been overlooked. Here we report a spectroscopic photon localization microscopy that is capable of capturing the inherent spectroscopic signatures of photons from individual stochastic radiation events. Spectroscopic photon localization microscopy achieved higher spatial resolution than traditional photon localization microscopy through spectral discrimination to identify the photons emitted from individual molecules. As a result, we resolved two fluorescent molecules, which were 15 nm apart, with the corresponding spatial resolution of 10 nm—a four-fold improvement over photon localization microscopy. Using spectroscopic photon localization microscopy, we further demonstrated simultaneous multi-colour super-resolution imaging of microtubules and mitochondria in COS-7 cells and showed that background autofluorescence can be identified through its distinct emission spectra.

Date: 2016
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms12290 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12290

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms12290

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().