In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum

Jakob Toudahl Nielsen (), Natalia V. Kulminskaya, Morten Bjerring, Juha M. Linnanto, Margus Rätsep, Marie Østergaard Pedersen, Petar H. Lambrev, Márta Dorogi, Győző Garab, Karen Thomsen, Caroline Jegerschöld, Niels-Ulrik Frigaard, Martin Lindahl and Niels Chr. Nielsen ()
Additional contact information
Jakob Toudahl Nielsen: Center for Insoluble Protein Structures (inSPIN)
Natalia V. Kulminskaya: Center for Insoluble Protein Structures (inSPIN)
Morten Bjerring: Center for Insoluble Protein Structures (inSPIN)
Juha M. Linnanto: Institute of Physics, University of Tartu
Margus Rätsep: Institute of Physics, University of Tartu
Marie Østergaard Pedersen: Center for Insoluble Protein Structures (inSPIN)
Petar H. Lambrev: Hungarian Academy of Sciences, Biological Research Centre
Márta Dorogi: Hungarian Academy of Sciences, Biological Research Centre
Győző Garab: Hungarian Academy of Sciences, Biological Research Centre
Karen Thomsen: Interdisciplinary Nanoscience Center (iNANO), Aarhus University
Caroline Jegerschöld: Karolinska Institutet
Niels-Ulrik Frigaard: Section for Marine Biology, University of Copenhagen
Martin Lindahl: Karolinska Institutet
Niels Chr. Nielsen: Center for Insoluble Protein Structures (inSPIN)

Nature Communications, 2016, vol. 7, issue 1, 1-12

Abstract: Abstract Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix.

Date: 2016
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DOI: 10.1038/ncomms12454

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