Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution

Krishna C Mudumbi, Eric C Schirmer and Weidong Yang ()
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Krishna C Mudumbi: Temple University
Eric C Schirmer: The Wellcome Trust Centre for Cell Biology, University of Edinburgh
Weidong Yang: Temple University

Nature Communications, 2016, vol. 7, issue 1, 1-6

Abstract: Abstract The normal distribution of nuclear envelope transmembrane proteins (NETs) is disrupted in several human diseases. NETs are synthesized on the endoplasmic reticulum and then transported from the outer nuclear membrane (ONM) to the inner nuclear membrane (INM). Quantitative determination of the distribution of NETs on the ONM and INM is limited in available approaches, which moreover provide no information about translocation rates in the two membranes. Here we demonstrate a single-point single-molecule FRAP microscopy technique that enables determination of distribution and translocation rates for NETs in vivo.

Date: 2016
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DOI: 10.1038/ncomms12562

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