RNF168 and USP10 regulate topoisomerase IIα function via opposing effects on its ubiquitylation

Kiran Kumar Naidu Guturi, Miyuki Bohgaki, Toshiyuki Bohgaki, Tharan Srikumar, Deborah Ng, Ramya Kumareswaran, Samah El Ghamrasni, Justin Jeon, Parasvi Patel, Mohamed Saad Eldin, Rob Bristow, Peter Cheung, Grant S. Stewart, Brian Raught, Anne Hakem () and Razqallah Hakem ()
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Kiran Kumar Naidu Guturi: Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto
Miyuki Bohgaki: Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto
Toshiyuki Bohgaki: Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto
Tharan Srikumar: Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto
Deborah Ng: Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto
Ramya Kumareswaran: Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto
Samah El Ghamrasni: Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto
Justin Jeon: Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto
Parasvi Patel: Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto
Mohamed Saad Eldin: Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto
Rob Bristow: Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto
Peter Cheung: York University
Grant S. Stewart: School of Cancer Sciences, University of Birmingham
Brian Raught: Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto
Anne Hakem: Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto
Razqallah Hakem: Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto

Nature Communications, 2016, vol. 7, issue 1, 1-13

Abstract: Abstract Topoisomerase IIα (TOP2α) is essential for chromosomal condensation and segregation, as well as genomic integrity. Here we report that RNF168, an E3 ligase mutated in the human RIDDLE syndrome, interacts with TOP2α and mediates its ubiquitylation. RNF168 deficiency impairs decatenation activity of TOP2α and promotes mitotic abnormalities and defective chromosomal segregation. Our data also indicate that RNF168 deficiency, including in human breast cancer cell lines, confers resistance to the anti-cancer drug and TOP2 inhibitor etoposide. We also identify USP10 as a deubiquitylase that negatively regulates TOP2α ubiquitylation and restrains its chromatin association. These findings provide a mechanistic link between the RNF168/USP10 axis and TOP2α ubiquitylation and function, and suggest a role for RNF168 in the response to anti-cancer chemotherapeutics that target TOP2.

Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12638

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DOI: 10.1038/ncomms12638

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