Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status

F. Coscia, K. M. Watters, M. Curtis, M. A. Eckert, C. Y. Chiang, S. Tyanova, A. Montag, R. R. Lastra, E. Lengyel () and M. Mann ()
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F. Coscia: Max Planck Institute of Biochemistry
K. M. Watters: Section of Gynecologic Oncology, University of Chicago
M. Curtis: Section of Gynecologic Oncology, University of Chicago
M. A. Eckert: Section of Gynecologic Oncology, University of Chicago
C. Y. Chiang: Section of Gynecologic Oncology, University of Chicago
S. Tyanova: Max Planck Institute of Biochemistry
A. Montag: University of Chicago Medicine
R. R. Lastra: University of Chicago Medicine
E. Lengyel: Section of Gynecologic Oncology, University of Chicago
M. Mann: Max Planck Institute of Biochemistry

Nature Communications, 2016, vol. 7, issue 1, 1-14

Abstract: Abstract A cell line representative of human high-grade serous ovarian cancer (HGSOC) should not only resemble its tumour of origin at the molecular level, but also demonstrate functional utility in pre-clinical investigations. Here, we report the integrated proteomic analysis of 26 ovarian cancer cell lines, HGSOC tumours, immortalized ovarian surface epithelial cells and fallopian tube epithelial cells via a single-run mass spectrometric workflow. The in-depth quantification of >10,000 proteins results in three distinct cell line categories: epithelial (group I), clear cell (group II) and mesenchymal (group III). We identify a 67-protein cell line signature, which separates our entire proteomic data set, as well as a confirmatory publicly available CPTAC/TCGA tumour proteome data set, into a predominantly epithelial and mesenchymal HGSOC tumour cluster. This proteomics-based epithelial/mesenchymal stratification of cell lines and human tumours indicates a possible origin of HGSOC either from the fallopian tube or from the ovarian surface epithelium.

Date: 2016
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DOI: 10.1038/ncomms12645

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